Extended Data Fig. 10: Pronuclear CRISPR Injections Targeting Gibbin Cause a Spectrum of Phenotypes in Mice.

(a) Quantification of three phenotype classes observed following Gibbin depletion in vivo. Mosaic genotypes exhibited CRISPR cutting on at least one allele. (b) Images of mouse embryos collected 18 days following pronuclear CRISPR injection to deplete Gibbin. Embryos were organized into four phenotypic classes. Injections were performed in duplicate across 6 different litters totaling n = 52 collected embryos. (c) Adult mice 5 weeks after receiving grafts from E18 mice. The GKO graft was taken from a more moderate mutant. n = 3 for each genotype, and grafting was repeated in two separate experiments. (d) H&E images depicting hair follicle cyst phenotypes from grafted mice. Images are representative of at least 3 biological and 3 technical replicates.