Extended Data Fig. 3: Gibbin and GATA3 are co-expressed.
From: Gibbin mesodermal regulation patterns epithelial development
(a) Principal component analysis (PCA) of RNA-seq from WT, GKO, TFAP2A KO, GATA3 KO, and p63 KO cells after 7 days of RA/BMP4 treatment. n = 2 biologically independent samples. (b) Violin plot of AHDC1/Gibbin or (b) GATA3 expression in each scRNA-seq cluster after 7 days of RA/BMP4. (c) Percent of cells after 7 days of RA/BMP4 which express AHDC1/Gibbin and/or GATA3. (d) qPCR of transcripts induced by GATA3 overexpression in WT or GKO backgrounds. Error bars are mean +/− s.d., n = 3 biologically independent samples averaged from 2 technical replicates. * indicates p-value < 0.05 by two-tailed Student’s t-test. (e) ChromHMM analysis of GATA3 binding sites showing enrichment of various chromatin states in relation to GATA3 ChIP–seq peaks. (f) Average distances between Gibbin and GATA3 ChIP–seq peaks.
