Extended Data Fig. 5: Impact of vaccine on functional programs of tumor-infiltrating CD4 and CD8 T-cells.
From: A vaccine targeting resistant tumours by dual T cell plus NK cell attack
a, Effect of doxycycline on tumor growth in vaccinated mice. Mice received Ctrl-vax (blue, grey) or MICB-vax (green, magenta, red) on day 0 and a boost on day 14. B16F10 cells transduced with Ctrl-dox or MICB-dox lentiviral vectors as indicated were implanted on day 21, and mice were treated with doxycycline (or PBS as control) starting on day 25 to induce MICB expression on tumor cells (n=5 mice/group). b–k, Analysis of tumor-infiltrating T-cell populations. Tumor-infiltrating T-cells were analyzed 7 days following induction of MICB expression by tumor cells (n = 7 mice/group). b–c, Analysis of CD62L and CD44 expression by tumor-infiltrating CD4 (b) and CD8 (c) T-cells. d–e, Representative flow plots (left) and quantification (right) of NKG2D receptor expression by CD4 (d) and CD8 (e) T-cells. f–g, Representative flow plots and quantification of CXCR6 receptor expression by CD4 (f) and CD8 (g) T-cells. h–i, Quantification of proliferating Ki67+ CD4 (h) and CD8 (i) T-cells. j–k, Quantification of TNFα positive CD4 (j) and CD8 (k) T-cells. Two-way ANOVA with Bonferroni’s post hoc test (a); two-way ANOVA with Tukey’s multiple comparison test (b–c); two-tailed Mann Whitney test (d–k). Representative data from two independent experiments (b–i); representative data from three independent experiments (j–k). Data depict mean+/− SEM.
