Extended Data Fig. 2: Aged lung conditioned medium promotes melanoma outgrowth in vitro.
From: Stromal changes in the aged lung induce an emergence from melanoma dormancy
a, b, FS13 c, d FS4 e, f FS14 g, h and 1205Lu GFP melanoma cells were seeded at a density of 2x104. Cells were then grown over a 10-day period in conditioned medium from young or aged lung or skin fibroblasts and underwent counting using a haemocytometer at days 3, 5 and 10 and were further assessed via automated counting using NIS elements. Experiment was performed in biological triplicates with 3 technical replicates each. A two-way ANOVA was performed with a post-hoc Holm-Sidak’s multiple comparisons test for each time point. P-values were taken from day 10. ****= P < 0.0001. ** P = 0.0084 for Fig. 2d. Data were presented as the mean +/− the SEM for each time point.
