Fig. 1: Plasma ELISAs and neutralizing activity.
From: Increased memory B cell potency and breadth after a SARS-CoV-2 mRNA boost
a, Graph showing area under the curve (AUC) for plasma IgG antibody binding to SARS-CoV-2 Wuhan-Hu-1 RBD after prime6, 1.3 months and 5 months after the second vaccination (Vax 2)5,6 and 1 month after the third vaccination booster (Vax 3) for n = 42 samples. Lines connect longitudinal samples. m, month. b, Graph showing anti-SARS-CoV-2 NT50 values of plasma measured by a SARS-CoV-2 pseudotype virus neutralization assay in 293TAce2 cells using wild-type (WT; Wuhan-Hu-1)32 SARS-CoV-2 pseudovirus16,33 in the plasma samples shown in a. c, Plasma neutralizing activity against the indicated SARS-CoV-2 variants of interest or concern for n = 15 randomly selected samples assayed in HT1080Ace2cl.14 cells. Wuhan-Hu-1 and Omicron BA.1 NT50 values were derived from ref. 7. Pseudoviruses in c were based on a spike protein that also included the R683G substitution, which disrupts the furin cleavage site and increases particle infectivity. All experiments were performed at least in duplicate. Red bars and values in a–c represent geometric mean values. Statistical significance in a and b was determined by two-tailed Kruskal–Wallis test with subsequent Dunn’s multiple-comparisons test. Statistical significance in c was determined by two-tailed Friedman test with subsequent Dunn’s multiple-comparisons test. P values are as indicated.
