Extended Data Fig. 6: Helicase domain of Dicer-2.
From: Structure of the Dicer-2–R2D2 heterodimer bound to a small RNA duplex
(a) Multiple sequence alignment of Dicer-2, RIG-I, Dicer-1, and human Dicer. The secondary structure of Dicer-2 is indicated above the sequences. The Hel1 and Hel2 domains are highlighted in light blue and blue, respectively. The key residues are marked by triangles. The figure was prepared using Clustal Omega (http://www.ebi.ac.uk/Tools/msa/clustalo) and ESPript3 (http://espript.ibcp.fr/ESPript/ESPript). (b, c) Helicase domains of Dicer-2 (b) and RIG-I (PDB: 3TMI) (c). CRD, carboxy-terminal regulatory domain. (d, e) ATP-binding sites of Dicer-2 (d) and RIG-I (PDB: 3TMI) (e). (f) Hydrophobic core in the Dicer-2 helicase domain. (g) Recognition of dsRNA by the Dicer-2 helicase domain. (h) Cryo-EM reconstitution of the Dicer-2 helicase domain bound to a blunt-end dsRNA (PDB: 6BU9). A RIG-I-based homology model was fitted to the 6.8-Å resolution density map as a rigid body.
