Extended Data Fig. 10: Recognition of the siRNA duplex by the Dicer-2–R2D2 heterodimer.
From: Structure of the Dicer-2–R2D2 heterodimer bound to a small RNA duplex
(a) Asymmetric recognition of the siRNA duplex by the Dicer-2–R2D2 heterodimer. (b) Recognition of the 1-nt 3′-overhang (G20) of the siRNA duplex by R2D2. U21 is disordered in the structure, and a possible location of U21 is indicated by a dashed circle. (c) Photocrosslinking experiments. Dicer-2 and R2D2 were incubated with 5′-radiolabeled siRNA containing 5-iodouracil at position 20 and a 0–2-nt 3′-overhang at the stable end. The reaction mixture was analysed by SDS-PAGE, and crosslinked proteins were detected using phosphorimaging (n = 3 independent experiments). (d) Possible interaction between R2D2 and a blunt-end siRNA. A modeled nucleotide complementary to G20 in the guide strand is indicated by an asterisk.
