Extended Data Fig. 6: Structure of preSSU1 state and accompanying RBFA.
From: Mechanism of mitoribosomal small subunit biogenesis and preinitiation
a, Superposition of rRNA shows the head rotation towards the A-site that exposes the P-site region. As a result of RBFA binding, uS7m is displaced (zoom in panels). b, The arrangement of RBFA in preSSU1 is incompatible with mS37 in SSU. It engages the C-terminal extension of bS6m in the binding, which is dislocated by 24 Å. The formed turn is just after a mitochondria-specific iron-sulfur cluster that is coordinated by bS6m and bS18m. c, RBFA:rRNA contacts with SSU. Arg166, His167, and Asn177 of RBFA interact with the rRNA 3′-end, which interacts with mS37 in the mature SSU. Thr132, Ser136, and Arg139 at β1-β2 loop of RBFA interact with the rRNA h28.
