Extended Data Fig. 2: Cryo-EM reconstruction of full-length, wild-type human GAT1 in complex with tiagabine.
a, Workflow of cryo-EM processing of GAT1 in complex with tiagabine in the inward-open conformation with representative micrograph, picking templates and 2D classes. Data processing was entirely performed in cryoSPARC57. After motion correction, CTF estimation and particle picking, the dataset was sorted using 2D classification, followed by 3D ab initio reconstruction and heterogenous refinement for further clean-up of the particle stack. Finally, 3D reconstructions were performed using non-uniform refinement58 and local refinement, which yielded a final map of 3.8 Å resolution. b, Data quality of the final reconstruction, illustrated as a local resolution map ranging from 3 - 4.8 Å, gold-standard fourier shell correlation plot (masked and unmasked) and angular sampling of the final reconstruction
