Extended Data Fig. 6: Glycoforms on IgG2 Fc conserved region N-glycans do not contain acetylated sialic acid.
From: Pregnancy enables antibody protection against intracellular infection
Lm-specific IgG from ΔActA Lm-primed virgin and pregnant mice was subjected to trypsin or chymotrypsin digestion and then LC-MS/MS analysis. (a) Representative MS/MS fragmentation from the conserved N-linked glycosylation site at position 183 on the Fc region of IgG2b/c (glycopeptide EDYNSTIR). (b) Extracted ion chromatograph of EDYNSTIR with the observed sialic acid (Neu5Gc) glycoforms and the absence of Acetyl-Neu5Gc, which was confirmed for the entire length of the LC-MS run. (c, d) All glycoforms with observed masses for N-glycans on the conserved Fc region of IgG2b/c for virgin (c) and pregnant (d) Lm-specific IgG. Experiment was performed in triplicate with representative (a, b) and combined (c, d) data shown.
