Extended Data Fig. 9: Deacetylated anti-Lm antibodies protect via CD22-mediated suppression of B cell IL-10 production.
From: Pregnancy enables antibody protection against intracellular infection
(a) Anti-Lm antibody titres in neonatal WT or CD22-deficient mice transferred sera from ΔActA Lm-primed pregnant/postpartum (pSera) or naive mice. (b, c) Bacterial burden (b) or anti-Lm IgG titres (c) after virulent Lm infection in neonatal mice transferred pSera or sera from naive mice along with anti-CD22 blocking or isotype control antibodies. (d, e) Bacterial burden (d) or anti-Lm IgG titres (e) after virulent Lm infection in neonatal mice transferred vIgG glycoengineered to express Neu5Ac. Neonates received either isotype control mAb or anti-CD22 mAb, or were CD22 deficient. (f, g) Bacterial burden (f) and anti-Lm IgG titres (g) after virulent Lm infection in WT or CD19-/- neonatal mice transferred vSera or sera from naive mice. (h) Anti-Lm IgG titres in neonatal WT or μMT-/- mice transferred sera from ΔActA Lm-primed virgin (vSera) or pSera. Each symbol represents an individual mouse, with graphs showing data combined from at least 2 independent experiments each with 3-5 mice per group per experiment. Bar, mean ± standard error. P values between key groups are shown as determined by one-way ANOVA adjusting for multiple comparisons. Dotted lines, limit of detection
