Extended Data Fig. 5: Oxonium ion searches for sialic acid modifications.
From: Pregnancy enables antibody protection against intracellular infection
(a) Schematic for purification of Lm-specific IgG from ΔActA Lm-primed virgin and pregnant mice for mass spectroscopy analysis. (b) Oxonium ion m/z used to search for sialic acid variants. Diagnostic ions were filtered through the MS/MS spectra to select glycopeptides containing Acetyl-Neu5Gc (Neu5Gc,Ac). Example spectra shown for virgin Lm-specific IgG. (c) MS/MS fragmentation for the low mass region (150-500) demonstrating sialic acid variants. All presented m/z are z = 1. Experiment was performed in triplicate with representative data shown.
