Extended Data Fig. 7: Pairwise correlation between replicates in estimated mutant fitness in each of the three additional environments used.
From: Synonymous mutations in representative yeast genes are mostly strongly non-neutral
a–c, Correlation between every two of the three replicates in estimated mutant fitness under SC at 37 °C. Each dot is a mutant and the dotted line indicates the diagonal. Pearson’s correlation r and its associated P-value are presented. Among-genotype sum of squares explains 96.1% of the total sum of squares (one-factor ANOVA). d–f, Correlation between every two of the three replicates in estimated mutant fitness under YPD + 0.375 mM H2O2. Among-genotype sum of squares explains 94.4% of the total sum of squares. g–i, Correlation between every two of the three replicates in estimated mutant fitness under YPE. j, Correlation between replicates 1 and 3 in estimated mutant fitness under YPE after the exclusion of SNF6 mutants. k, Correlation between replicates 2 and 3 in estimated mutant fitness under YPE after exclusion of SNF6 mutants. Panels g-k suggest that the fitness estimates of SNF6 mutants in replicate 3 under YPE are unreliable, so are unused in fitness estimation in YPE. When SNF6 is excluded, among-genotype sum of squares explains 91.0% of the total sum of squares in YPE
