Extended Data Fig. 15: Granzyme B, active caspase-3 and active caspase-7 are not sufficient to kill bacteria in vitro.
From: Caspase-7 activates ASM to repair gasdermin and perforin pores
a–d, NK co-culture killing assays (see “In vitro co-culture assays” in methods for detailed description) with C. violaceum-infected YAC-1 cells or L. monocytogenes-infected Hepa1-6 and YAC-1 cells as indicated. (a, c) Western blot analysis of cleaved caspase-3 (CC3) and/or cleaved caspase-7 (CC7). (b, d) Bacterial counts 5 h post co-culture. e-f, Hepa1-6 cell lysates were spiked with granzyme B at the indicated amounts for 1 h and 8x106 L. monocytogenes was added. Western blot analysis of CC3 and CC7 (e) and time course of bacterial numbers (f). g, Invasion assay with L. monocytogenes incubated in Hepa1-6 cell lysates for 16 h with granzyme B. All data are representative of 2 experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (Two-sided Mann–Whitney U-test). Bars indicate mean
