Fig. 3: Nascent (pentapeptide-rich) and mature (tetrapeptide-rich) PG differentially affect BAM activity.
From: Peptidoglycan maturation controls outer membrane protein assembly
a, Schematic representation of the PG structure in MC1061 and CS703-1, which are enriched in tetrapeptides (tetra-rich) and pentapeptides (penta-rich), respectively. b, Interaction of BamABCDE with tetrapeptide-rich and pentapeptide-rich sacculi in vitro after pull-down by SDS–PAGE and Coomassie blue staining (gel source data are shown in Supplementary Fig. 1). No PG, negative control without PG. c–e, Dose-dependent effects of tetrapeptide-rich (left) and pentapeptide-rich (right) PG on BAM-mediated OmpT assembly in vitro. Data are mean ± s.d. of three independent experiments.
