Extended Data Fig. 3: OMP biogenesis is septal biased and cell cycle dependent in native expression systems and division mutants. | Nature

Extended Data Fig. 3: OMP biogenesis is septal biased and cell cycle dependent in native expression systems and division mutants.

From: Peptidoglycan maturation controls outer membrane protein assembly

Extended Data Fig. 3

(A) Co-labelling of BamA and FepA with αBamAAF488 and ColB-mCherry, respectively, after 7 min induction of FepA biogenesis. Shown is an overlay of the BamA (green) and FepA (red) channels. (B) Timeline of FepA biogenesis following native chromosomal expression under iron limiting conditions. Samples were taken and labelled with ColB-GFP at different time points after the addition of 2,2 Bipyridyl to LB growth media. (C) Fluoresence intensity of cells shown in panel B. (D) Epifluorescence images of FepA in a ΔminB strain (PB114). Shown are images of cells before and after chromosomal induction with 2,2 Bipyridyl. Scale bars, 1 μm.

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