Extended Data Fig. 2: SHOC2:PP1C:RAS complex functional validation.
From: Structural basis for SHOC2 modulation of RAS signalling
a, SPR data showing that KRAS(GNP) associates with SHOC2:PP1C, but not KRAS(GDP). b, Nucleotide bound KRAS does not show binding to PP1C alone nor c, SHOC2 alone, showing that binding in a is due to RAS binding the SHOC2:PP1C complex. d, SEC traces (top) showing SHOC2 does not associate with KRAS(GCP) (green) or MRAS(GCP) (orange) in the absence of PP1C, and associated SDS-PAGE analysis of SEC fractions (bottom). Results representative two independent experiments. e, Representative plot of dephosphorylation of BRAF NTpS phosphopeptide by 10 nM PP1C or 10 nM PP1C + 1 µM SHOC2 + 10 µM KRAS(GCP). Data points show 2 and 4 min time points (n = 2).
