Extended Data Fig 4: Cryo-EM and x-ray structure comparisons.
From: Structural basis for SHOC2 modulation of RAS signalling
a, Unsharpened cryoEM map of SHOC2:PP1C:MRAS complex contoured to 0.15 (left). Sharpened cryoEM map of SHOC2:PP1C:MRAS complex contoured to 0.35 (sharpening B factor = −110 Å2)(right). Density is not observable for the SHOC2 C-terminus at these contours. b, Example model and sharpened density of MRAS SwII region. c, left, PP1C from cryoEM structure (purple) aligned with PP1C x-ray structure (PDB: 4MOV). Right, MRAS(GCP) from cryoEM structure (salmon) aligned with MRAS(GNP) x-ray structure (PDB: 1X1S). d, Electrostatic view of SHOC2 and PP1C showing basic and acidic regions (blue/white/red) with aligned model of prenylated MRAS model (based on PDB code 5TAR) (yellow), looking down from the membrane, which is parallel to the surface of the page. e, X-ray crystal structure of SHOC2 (orange, left) with electrostatic view showing basic and acidic regions (blue/white/red, right). f, Alignment of SHOC2:PP1C:MRAS cryoEM structure (green:purple:salmon) with SHOC2 x-ray structure (orange), performed using the N-terminal cap and first four LRRs of SHOC2. A 9° twist is evident between both structures relative to the 1st LRR.
