Extended Data Fig. 4: Effect of cGAS on differential expression of reversine-treated BT549 cells.
From: cGAS–STING drives the IL-6-dependent survival of chromosomally instable cancers
a) Differential expression of a parental BT549 cell line upon 48h of treatment with reversine vs. DMSO. X-axis shows log2 fold change, y-axis FDR-adjusted p-values. Size of the circle corresponds to the expression level in the untreated condition. Biological processes are characterised as MSigDB Hallmark (middle) and DoRothEA transcription factor target genes (right). Significance of gene sets was tested comparing the Wald statistic of genes within vs. genes outside the set using a linear model. Size of the circle corresponds to the number of genes in the set. b) Differential expression of cGASKO BT549 cells reversine vs. DMSO shows a clearly diminished transcriptional response, indicating that most of the transcriptional response to CIN is driven by cGAS, characterised by a drop of interferon response genes (middle) and STAT1/NF-kB-responsive genes. c) Differential expression of reversine-treated BT549 cells between cGASKO and wild type (parental) confirms a lower expression of interferon and STAT1-driven genes in the knockout vs. the wild type condition.
