Fig. 1: PIEZO1 is expressed in mouse and human putative itch receptors.

a–c, Representative images (five images from two mice) of sectioned mouse DRG smFISH for Piezo1 (a), Nppb (b) and merged with DAPI (c). White arrowheads indicate Piezo1⁺ or Nppb⁺ cells. d, Quantification of mouse DRG smFISH images showing the percentage of cells expressing a given marker (bar labels) that were co-labelled with Piezo1 transcript. The number of analysed neurons is indicated above the bar (four to six images per marker from two mice). e, Data from d presented as the percentage of Piezo1⁺ neurons that co-express a given marker. f, Percentage of Nppb⁺ neurons expressing Piezo1 or Piezo2. g, Comparison of Piezo2 expression in Nppb⁺ versus Mrgpra3⁺ neurons. h, Quantification of human smFISH images (seven to eight images per marker from one donor; see Extended Data Fig. 4a,b). i–l, Representative images of sectioned mouse DRGs labelled with antibodies against tdTomato (images show PIEZO1 (i), PECAM1 (j), neurofilament H (NEFH; k) and the merged image (l)). Asterisks indicate blood vessels and arrowheads indicate a PIEZO1⁺ neuron and nerve fibre. The experiment was repeated one additional time. All images are presented as maximum intensity z-projections of confocal images. Scale bars, 100 µm.