Extended Data Fig. 7: Additional somatosensory behaviours in Piezo1 fl/fl;PirtCre mice.
a, Histamine-evoked itch (Kruskal–Wallis: *p = 0.0386; χ2 = 6.510; Dunn’s: *padjusted = 0.0216). b, Total scratch bouts following histamine (Kruskal–Wallis: *p = 0.0296; χ2 = 7.043; Dunn’s: *p = 0.0165). c, Mean bouts per episode from a (Kruskal–Wallis: **p = 0.0047; χ2 = 10.73; Dunn’s: **padjusted = 0.0021). d, Scratch episodes following histamine (Kruskal–Wallis: p = 0.9401; χ2 = 0.1234). a–d are from n =11 WT, n = 6 HET, and n = 8 KO mice from 3 experiments. e, Chloroquine-evoked itch (Mann–Whitney: p = 0.8078; U = 50, n = 12, 9). f, Total scratch bouts following chloroquine (Mann–Whitney: p = 0.6511; U = 47). e-f are from n = 12 WT and n = 9 KO mice from 2 experiments. g, IL-31-evoked itch (Mann–Whitney: ***p = 0.0008; U = 16.50, n = 11, 13). h, Total scratch bouts following IL-31 (Mann–Whitney: ***p = 0.0009; U = 17). i, Mean bouts per episode from g (Mann–Whitney: ****p < 0.0001; U = 0.5). j, IL-31-evoked alloknesis (Mann–Whitney: ****p < 0.0001; U = 0). g-i are from n = 11 WT and n = 13 KO mice from 2 experiments. k, Ear model of mechanical itch (Two-way ANOVA: ***pgenotype = 0.0002; F(2, 22) = 12.93; Sidak’s padjusted: *p0.16g = 0.0214; ****p0.4g < 0.0001, n = 11 WT, n = 6 HET, and n = 8 KO mice from 3 experiments). l, % withdrawal response (Two-way ANOVA: ***pgenotype = 0.0007; F(2, 29) = 9.524; Dunn’s **padjusted = 0.0014). m, 50% withdrawal von Frey threshold (Kruskal–Wallis: ***p = 0.0002; χ2 = 16.71; Dunn’s: ***p = 0.0007). n, Pinprick response (Kruskal–Wallis: p = 0.2568; χ2 = 2.719). o, Pinprick latency (Kruskal–Wallis: p = 0.3500; χ2 = 2.100, N = 11 WT, N = 6 HET, and N = 8 KO mice from 3 experiments). p, Randall–Selitto (Kruskal–Wallis: p = 0.2225; χ2 = 3.006). q, Tail clip (Kruskal–Wallis: p = 0.2505, χ2 = 2.769). Data in l–n and p,q are from n = 16 WT, n = 6 HET, and n = 10 KO mice from 4 experiments. r, Proprioception scores (Mann–Whitney: p = 0.6430, U = 57, n = 10, 13 mice from 1 experiment). Error bars represent mean ± s.e.m. of n biological replicates (mice) and statistical tests are two-tailed where applicable.
