Extended Data Fig. 9: Assessment of interestial pressure, immunosurveillance and apoptosis in CTCs at different timepoints of the circadian rhythm.
From: The metastatic spread of breast cancer accelerates during sleep
a, Representative immunofluorescence images of Taz and Yap in CTCs collected at ZT4 and ZT16 from NSG-LM2 and NSG-CDX-BR16 mice. Plots showing the distribution of Taz and Yap intensity in CTCs shown in the same panel (n = 3). Scale bar = 10 μm. b, Plot showing the expression distribution of TEAD genes in CTCs from NSG-CDX-BR16 mice. The fold change (FC, in log2 scale) and adjusted P value from the global differential expression analysis are shown for each gene. c, Gating strategy to determine the frequency of the indicated cell populations in panels “d” and “e”. The percentage values refer to the parental population considered in each panel. d, Plot showing the frequency of white blood cells (WBCs) from peripheral blood (PB) isolated during the rest (n = 8) or active phase (n = 10) from BALB/c-4T1 mice. e, Plot showing the frequency of tumor-infiltrated WBCs isolated during the rest (n = 8) or active phase (n = 10) from BALB/c-4T1 mice. f, Representative immunofluorescence images of cleaved caspase-3 in CTCs collected at ZT4 and ZT16 from the NSG-LM2 and NSG-CDX-BR16 mice. Plots showing the distribution of cleaved caspase-3 intensity in CTCs shown in the same panel (n = 3). Scale bar = 10 μm. For panels “a” and “f”, center lines in the box represent the median; box limits represent first and third quartile; extremes of the whisker lines represent the minimum and maximum observed values. ns: non statistically significant by two-sided Mann-Whitney test. For panels “d” and “e”, data are presented as mean ± s.e.m.; ns: non statistically significant by unpaired two sided t-test. For all panels, n represents the number of biologically independent mice
