Extended Data Fig. 1: Tumor size and CTCs intravasation rates during different phases of the circadian rhythm.
From: The metastatic spread of breast cancer accelerates during sleep
a, Pie charts displaying the mean percent of total CTCs, single CTCs, CTC clusters and CTC-WBC clusters detected during the rest or active phase in breast cancer patients (n = 30), in NSG-LM2 mice (n = 6), NSG-CDX-BR16 mice (n = 6), NSG-4T1 mice (n = 4) or BALB/c-4T1 mice (n = 6). b, Time kinetic analysis showing mean CTC counts in the NSG-LM2 (n = 3), NSG-4T1 (n = 3) and BALB/c-4T1 (ZT0, ZT4, ZT20 n = 3; ZT12, ZT16 n = 4) breast cancer mouse models over a 24-h time period. Data are presented as mean ± s.e.m. c, Plots showing the size of the primary tumors dissected at different timepoints from NSG-CDX-BR16 mice (ZT0, ZT4, ZT16, ZT20 n = 4; ZT12 n = 3), NSG-LM2 (n = 3), NSG-4T1 mice (n = 3) and BALB/c-4T1 (ZT0, ZT4, ZT20 n = 3; ZT12, ZT16 n = 4) mice. Data are presented as mean ± s.e.m. d, Representative bioluminescence images of lungs from NSG-CDX-BR16, NSG-LM2 and NSG-4T1 mice taken at different timepoints (ZT0, ZT4, ZT12, ZT16, ZT20) (n = 3). e, Box plots showing the distribution of the number of single CTCs (P = 0.0043), CTC clusters (P = 0.0087) and CTC-WBC clusters (P = 0.0130) collected at ZT4 or ZT16 in the NSG-CDX-BR16 mouse model (n = 6). Center lines in the box represent the median; box limits represent first and third quartile; extremes of the whisker lines represent the minimum and maximum observed values. Data are presented as mean ± s.e.m.; * P < 0.05, ** P < 0.01 by two-sided Mann-Whitney test. f, Plots showing the size of primary tumors from NSG-CDX-BR16 (n = 6), NSG-LM2 (n = 6), NSG-4T1 (n = 4) and BALB/c-4T1 (n = 5) mice dissected at ZT4 or ZT16. Data are presented as mean ± s.e.m. g, Plots showing the mean fold change increase of CTC counts isolated at ZT4 or ZT16 from NSG-LM2, NSG-CDX-BR16, NSG-4T1 and BALB/c-4T1 mice. h, Pie charts displaying the mean percentage of single CTCs, CTC clusters and CTC-WBC clusters detected during the rest or active phase in patients (n = 7), NSG-LM2 (n = 6), NSG-CDX-BR16 (n = 6), NSG-4T1 (n = 4) or BALB/c-4T1 (n = 6) mice. For all panels, n represents the number of biologically independent mice
