Extended Data Fig. 2: The abundance of CTCs during the rest phase is due to increased intravasation.

a, Box plots showing the distribution of the number of CTCs collected at ZT4 or ZT16 via cardiac puncture or tumor draining vessel (TDV) in the NSG-CDX-BR16 breast cancer mouse model (n = 4; P = 0.0286 for all). b, Representative bioluminescence images of lungs from NSG-CDX-BR16 mice taken at different timepoints (ZT0, ZT4, ZT12, ZT16, ZT20) over a 24-h time period (n = 4). c, Box plots showing the distribution of the number of CTCs collected at ZT4 or ZT16 via cardiac puncture or TDV in the NSG-LM2 breast cancer mouse model (n = 4; P = 0.0286 for all). d, Representative bioluminescence images of lungs from NSG-LM2 mice taken at ZT4 or ZT16 (n = 4). e, Plot showing the size of primary tumors dissected from NSG-LM2 mice at ZT4 or ZT16 (n = 4). f, Time kinetic analysis showing fold change differences in the number of LM2 cells detected in the circulation after their intravascular inoculation at different time points of the circadian rhythm (ZT0, ZT4, ZT12, ZT16) (n = 3 except ZT4 where n = 4). g, Plots showing the percentage of CTC clearance at different time points of the circadian rhythm (ZT0, ZT4, ZT12, ZT16) 5 min after intravascular inoculation of LM2 cells (n = 3 except ZT4 where n = 4). For panels “e”, “f” and “g”, data are presented as mean ± s.e.m. For panels ‘a’ and “c”, center lines in the box represent the median; box limits represent first and third quartile; extremes of the whisker lines represent the minimum and maximum observed values. * P < 0.05 by two-sided Mann-Whitney test. For all panels, n represents the number of biologically independent mice

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