Extended Data Fig. 7: FACS gating strategies.
From: The gut metabolite indole-3 propionate promotes nerve regeneration and repair
a. Gating strategy for the quantification of Ly6G+hi neutrophils (as % of CD45+ and total cell counts) from the nerve crush site following IPA or PBS treatment (corresponding to Extended Data Fig. 6i–k). b. Gating strategy for the verification of neutrophil depletion following αLy6G or αIgG2A treatment (corresponding to Extended Data Fig. 8h, i). Gr-1+ neutrophils were quantified from the spleen as percentage of CD45+.
