Extended Data Fig. 10: Increased number of CXCR2+ neutrophils following IPA treatment and depletion with anti-CXCR2 antibody in nerve tissue.
From: The gut metabolite indole-3 propionate promotes nerve regeneration and repair
a. Representative images of CXCR2+Ly6G+ cells in DRG tissue. Scale bar: 20 µm. b. Quantitative analysis of CXCR2+Ly6G+ cells in DRG tissue (N = 4 biologically independent animals per group, two-sided Student t-test, p-value compares CXCR2+Ly6G+ groups, data are presented as mean values +/ − SEM). c. Representative images of Ly6G immunostaining in sciatic nerve sections (crush site) of mice treated with IPA or PBS and anti-CXCR2 or anti-IgG monoclonal antibody 3 days after SNC. Scale bar: 250 µm. Scale bar in magnification: 50 µm. d. Quantification of the number of Ly6G+ cells in the sciatic nerve crush site of mice treated with IPA or PBS and anti-CXCR2 or anti-IgG monoclonal antibody 3 days after SNC (N = 4 biologically independent animals per group with a bilateral sciatic nerve crush; data are presented as mean values +/− SEM).
