Extended Data Fig. 1: The SA pathway is downregulated at elevated temperatures in different plant species examined. | Nature

Extended Data Fig. 1: The SA pathway is downregulated at elevated temperatures in different plant species examined.

From: Increasing the resilience of plant immunity to a warming climate

Extended Data Fig. 1: The SA pathway is downregulated at elevated temperatures in different plant species examined.

a–b, SA levels in 4-week-old Col-0 plants at 24 h after treatment [i.e., 1 day post-inoculation (dpi)] with flg22 peptide treatment (a) or Pst DC3000(avrRps4) inoculation [1.0 x 108 Colony Forming Units (CFU) mL−1] (b) at 23 °C and 28 °C. c–d, Transcript levels of BnaPR1 in leaves of 4-week-old rapeseed Westar plants infiltrated with mock (0.25 mM MgCl2) or Pst DC3000 [1.0 x 105 Colony Forming Units (CFU) mL−1] (c) and NtPR1 in leaves of 4-week-old tobacco plants infiltrated with mock (0.25 mM MgCl2) or Ps tabaci 11528 [1.0 x 106 Colony Forming Units (CFU) mL−1] (d) at 24 h post-inoculation (1 dpi) at 23 °C and 28 °C. e, BnaPR1 expression levels in leaves of 4-week-old rapeseed Westar plants 1 day after mock (0.1% DMSO) or 50 µM BTH treatment at 23 °C and 28 °C f, SA marker gene (SlPR1b) expression levels in 4-week-old Castlemart tomato plants 1 day after mock (0.1% DMSO) or 100 µM BTH treatment at 23 °C and 32 °C. g, SA marker gene (OsPR1b) expression levels in 5-week-old rice plants 1 day after mock (0.1% DMSO) or 200 µM BTH treatment at 28 °C and 35 °C. Results show the means ± S.D. [n = 3 (c, eg) or 4 (a, b, d) biological replicates] from one representative experiment (of three independent experiments) analyzed with two-way ANOVA with Tukey’s HSD for significance. Exact P-values for all comparisons are detailed in the Source Data files

Source data

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