Extended Data Fig. 5: Comparison with results in transgenic mice.

a, Top row: modulation of visual responses by running vs. correlation to running speed during spontaneous behaviour, for Pvalb, Sst, and Vip interneurons identified in transgenic mouse lines. Data re-analysed from Ref. ³⁰ and including 4 new mice. Bottom row: same analysis using interneurons identified by post-hoc transcriptomic analysis (data from this study; the Vip group included Vip-positive Sncg cells which are likely to be labelled in the Vip-Cre transgenic line). b, Size tuning curves of Vip, Pvalb and Sst cells for both datasets. Top row: responses measured in transgenic mice for centred stimuli (0-10° offset from receptive field centre); second row: response to off-centre stimuli (10-20° offset from receptive field) in transgenic mice; bottom two rows, same from post-hoc transcriptomics. Orange curves: responses during running; blue curves, responses during stationary epochs. Numbers at the top right corner of each plot indicate number of cells. Data are given as mean ± s.e.m. c, Classification of cell type from physiological features was identical for the two cell typing methods. Each cell was assigned to either Sst, Pvalb or Vip based on 14 physiological features (such as correlation to running speed, size tuning curves, skewness), using one of 3 different linear classifiers trained on a training set randomly selected from the transgenic recording sessions. Left: training-set classifier accuracy averaged over multiple random selections of the training set. Centre: accuracy of the classifiers averaged over the held-out transgenic sessions (test sets). Right: out-of-sample accuracy of the linear models on data with interneurons identified by post-hoc transcriptomics. Note the similar performance on transgenic and transcriptomic test sets. Error bars: s.d. over divisions into training and test set.