Fig. 6: Correlation of state modulation with cellular properties.
From: A transcriptomic axis predicts state modulation of cortical interneurons
a, Correlation between state modulation and electrophysiological properties measured by an independent Patch-seq study7. Each symbol represents mean values for a subtype, coded as in Fig. 5b. Rheobase: r = −0.63, P = 5 × 10−5; spike adaptation: r = 0.70, P = 3 × 10−6; spike shape index: r = 0.49, P = 0.003; time constant: r = 0.57, P = 4 × 10−4 (significance, Pearson correlation). b, Correlation between state modulation and cholinergic receptor expression obtained from an independent scRNA-seq study3. Each symbol represents mean values for a given subtype, coded as before. Chrm4: r = −0.50, P = 0.002; Chrm3: r = 0.63, P = 5 × 10−5; Chrna4: r = 0.52, P = 0.0014; Chrna5: r = 0.37, P = 0.03. Correlations of state modulation with excitatory cholinergic receptor expression were higher than with inhibitory receptor expression (including receptors not shown here; P = 0.008, F(1) = 12.2, two-sided ANOVA; only receptors with more than 2 counts in at least 5 subtypes were considered, making 10 in total). *P < 0.05, **P < 0.01, ***P < 0.001; black lines are linear regression fits. c, Schematic summarizing the transcriptomic axis and its functional and cellular correlates. Right, schematic of inputs from inhibitory neurons along the transcriptomic axis to a layer-2/3 (L2/3) cortical excitatory cell. ACh, acetylcholine.
