Extended Data Fig. 10: RALF37 triggers synergid Ca2+ changes and enhances the activity of NTA trio.
From: A receptor–channel trio conducts Ca2+ signalling for pollen tube reception
a, Representative Ca2+ spiking patterns in synergid cells in response to 0.5 µM RALF37. b, The peak values of Ca2+ spiking as in (a). n = 18 ovules for water treatment and n = 14 ovules for RALF37. c, d, Representative cytosolic Ca2+ spiking curves (c) and statistical analysis of peak values (d) in COS7 cells expressing the FER-LRE-NTA trio treated with 0.5 µM RALF37. n = 8 replicates, and ~ 60 cells were imaged in each duplicate. Error bars depict means ± S.E.M. All P values were determined by two-tailed Student’s t-test.
