Extended Data Fig. 2: RALF8, 9, 15, 25, 26 and 30 failed to induce synergid Ca2+ changes.
From: A receptor–channel trio conducts Ca2+ signalling for pollen tube reception

a, Representative Ca2+ spiking patterns in synergid cells in response to 0.5 µM RALFs. b, The peak values of Ca2+ spiking as in (a). n = 15 ovules for water treatment, n = 16 ovules for RALF8, n = 14 ovules for RALF9, n = 10 ovules for RALF15, n = 15 ovules for RALF25, n = 11 ovules for RALF26 and n = 13 ovules for RALF30. Error bars depict means ± S.E.M. All P values were determined by two-tailed Student’s t-test.