Extended Data Fig. 7: Cryo-EM structure determination of PolαPrim.
From: Structure of Tetrahymena telomerase-bound CST with polymerase α-primase
a, Data processing workflow (detailed in Methods). b, Representative 2D-class averages of PolαPrim particles obtained from cryo-EM images. The two classes shown in Fig. 3e are labelled with red boxes. c, Resolution of final reconstructions determined by gold-standard FSC at the 0.143 criterion. d, Euler angle distributions of particles used for the final reconstructions. e, Local resolution evaluations of the final reconstructions. f, FSC coefficients as a function of spatial frequency between model and cryo-EM density maps. The composite map is generated using Phenix69 with two focused refined maps (detailed in Methods). For the full map and the composite map, the complete model was used to calculate the FSCs. For the two focused refined maps, only corresponding regions of the model were used to calculate the FSCs. g, Representative cryo-EM densities (grey mesh) encasing the related atomic models (coloured sticks and ribbons).
