Extended Data Fig. 5: Amnion segregation from the embryonic disc.
From: Spatial profiling of early primate gastrulation in utero
a, PCA of EmDisc and Am based on the top 5000 most variable genes, PC1 = 20.7%, PC2 = 13.6%. b, Marker expression delineates the divergence of EmDisc and Am. Genes enriched in EmDisc and Am are marked in blue and purple, respectively; preimplantation genes are depicted in green. Stream plot track width is scaled to relative expression normalized to the mean across all stages displayed. c, Heatmap of expression of differentially expressed genes (DEG) in embryonic and extraembryonic lineages displayed in (a, b). Representative genes (left panel) and key gene ontology (GO) enrichment analysis (right panel) are shown. Genes shown in heatmap from Seurat function FindAllMarkers (minimum percent 50%, minimum log fold change 0.25) and filtered by adjusted p-value < 0.05. d–i, Virtual cross-sections of 3D-transcriptomes at CS5, 6 and 7 depicting mRNA levels of representative genes for (d) pluripotency factor expression in the nascent Am, (e) Am-Tb shared genes, (f) Am-ExMes shared genes, (g) Am-specific genes, (h) epithelial genes, (i) ECM-related genes. Categories indicated on the left of each panel. EmDisc, embryonic disc; Am, amnion.
