Extended Data Fig. 6: Flies prefer to lay eggs on leucine-containing food in a fashion that requires the leucine-binding capacity of Sestrin.
From: Sestrin mediates detection of and adaptation to low-leucine diets in Drosophila
a, Schematic of the setup used in the egg-laying preference assay. Two identical apple pieces were painted with solutions containing different substances and placed on opposite sides of a container. Animals were allowed to feed ad libitum over the course of the assay and the number of eggs deposited on each apple was counted after 24 h. b, c, Wild-type flies prefer to lay eggs on yeast- or amino acid-painted apples over water-painted apples. Scale bars, 1 mm. d-h, SesnL431E and Sesn−/− animals do not prefer to lay eggs on the leucine-containing apple. (a) created with BioRender.com. Values are mean ± SD of three biological replicates from a representative experiment. Data are representative of two independent experiments with similar results. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test (d-g), and Šídák's multiple comparisons test (h).
