Extended Data Fig. 2: The SesnL431E mutation does not affect adult fly lifespan on the chemically defined diets but does mildly delay larvae development, while loss of Sestrin does not affect larvae development.
From: Sestrin mediates detection of and adaptation to low-leucine diets in Drosophila
a, Loss of Sestrin does not the affect development of larva feeding on a complete diet. Time to pupariation for w1118 and Sesn−/− larvae fed the standard yeast-based diet. b-g, Survival curves for animals of the indicated sex and genotypes fed the indicated chemically-defined diets. (a) nWT(OreR)=235; n(SesnL431E)=238; (b) nWT(OreR)=233; n(SesnL431E)=237; (c) nWT(OreR)=242; n(SesnL431E)=248; (d) nWT(OreR)=242; n(SesnL431E)=240; (e) nWT(OreR)=229; n(SesnL431E)=243; (f) nWT(OreR)=245; n(SesnL431E)=245. See statistics in Supplementary Data 1 and methods. h. SesnL431E larvae raised on a standard yeast-based diet are developmentally delayed. Data are representative of three independent experiments with similar results. Statistical analysis was performed using a permutation test on the difference of the mean pupariation times of the two genotypes (a, h).
