Extended Data Fig. 9: Overview of HDCR subtomogram averaging, and helical pitch comparison between in vitro and in situ structures. | Nature

Extended Data Fig. 9: Overview of HDCR subtomogram averaging, and helical pitch comparison between in vitro and in situ structures.

From: Membrane-anchored HDCR nanowires drive hydrogen-powered CO2 fixation

Extended Data Fig. 9: Overview of HDCR subtomogram averaging, and helical pitch comparison between in vitro and in situ structures.The alternative text for this image may have been generated using AI.

a, Processing flowchart used for HDCR subtomogram averaging in situ. For additional details, see Methods. b, Fourier shell correlation (FSC) curves from the final subtomogram average (displayed in Fig. 5g). c, comparison of observed helical pitch in vitro (98.5 nm with rise: 68.4 Å, twist: 25°) and in situ (289.5 nm with rise: 67.8 Å, twist: 8.43°).

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