Extended Data Fig. 2: Filament bundling of purified HDCR used for cryo-EM and negative staining.
From: Membrane-anchored HDCR nanowires drive hydrogen-powered CO2 fixation
a–d, Longer HDCR filaments were occasionally observed in cryo-EM micrographs of the purified HDCR preparation. These filaments generally grouped together as bundles with varying filament length, impeding structural analysis. Representative images from 33,853 micrographs collected. Micrograph recording was performed as described in Extended Data Fig. 1. Scale bar: 50 nm. e–f, Representative negative-stain images of HDCR_His from F2 of Fig. 3f (n = 8), showing large filament bundles. Scale bars: 100 nm.
