Extended Data Fig. 12: DNA FISH targeting EHD2 locus.
From: Spatially resolved clonal copy number alterations in benign and malignant tissue
Representative images from fresh frozen squamous cell carcinoma tissue sections labelled with Cytocell 19q13/19p13probe. Consecutive sections were used for H&E, DAPI staining and FISH. Control probe labelled the 19p13.2 region of chromosome 19 in green, and EHD2 is shown in red. Nuclei counterstained with DAPI (dark blue). Yellow dashed rectangles mark the clonal group B position 1 and 2. Red dashed rectangles mark the clonal group A positions 1, 2, and 3. Blue dashed rectangles mark the clonal group D positions 1, and 2. Green rectangle marks the clonal group C position 1. Predicted deletions of EHD2 gene are shown in clones A, B, C, and D. Note that the clonal groups C, and D show deletions of EHD2 gene as well as diploid cells.
