Extended Data Fig. 4: Neutrophil depletion results in significant CFU burden in skin at 72 h post-infection.
From: A monocyte–leptin–angiogenesis pathway critical for repair post-infection
a–c, C57/BL6 mice were treated with neutrophil depleting antibody (LY6G 1A8) or isotype control antibody (2A3) and infected with low-dose S. aureus bead. a, Quantification of bacterial CFUs from skin at 24 and 72 h. n = 5 mice per time point from 2 independent experiments; two-way ANOVA with Šídák’s multiple comparisons test; data are mean ± s.e.m.; P = 0.9335 (24 h 2A3 vs 24 h 1A8), P = 0.0038 (72 h 2A3 vs 72 h 1A8), P = 0.6611 (24 h 2A3 vs 72 h 2A3), P = 0.0076 (24 h 1A8 vs 72 h 1A8). b, Quantification of abscess size at 24 and 72 h. n = 5 mice per time point from 2 independent experiments; two-way ANOVA with Šídák’s multiple comparisons test; data are mean ± s.e.m.; P = 0.9831 (24 h 2A3 vs 24 h 1A8), P = 0.0062 (72 h 2A3 vs 72 h 1A8), P = 0.8751 (24 h 2A3 vs 72 h 2A3), P = 0.0043 (24 h 1A8 vs 72 h 1A8). c, Representative image of a 72 h infection in isotype or 1A8-treated mouse.
