Extended Data Fig. 13: Metabolic profile of Adipo-Appbp2 KO mice on HFD.
From: Post-translational control of beige fat biogenesis by PRDM16 stabilization
a, Tissue weight at 12 weeks of HFD. n = 10 (Adipo-Appbp2 KO), n = 11 (Control). b, Relative mRNA levels of indicated genes in the IngWAT of mice in Fig. 5f. c, H&E staining in the iBAT of mice in Fig. 5f. Scale bar, 210 μm. Representative images from two biologically independent samples per group. d, Oleic acid oxidation in the indicated tissues at 10 weeks of HFD. n = 4 per group. e, f, AOC of in Fig. 5g (e) and Fig. 5h (f). g, Glucose tolerance test at 4 weeks of HFD. n = 10 per group. h, AOC of g. i, Insulin tolerance test at 5 weeks of HFD. n = 10 per group. j, AOC of i. k, l, Immunoblotting of phosphorylated (S473) and total Akt protein in the liver, EpiWAT and IngWAT at 4 weeks of HFD. n = 7 (Adipo-Appbp2 KO), n = 7 (Control) relative to HSP90 (k). Right: Quantification of phosphorylated (S473) AKT protein levels normalized to total AKT protein levels. n = 5 per group (l). m, Relative mRNA levels of indicated genes in the IngWAT at 4 weeks of HFD. n = 9 (Adipo-Appbp2 KO), n = 10 (Control). n, Heat-map of indicated genes in the epididymal WAT at 12 weeks of HFD. o, Relative mRNA levels of indicated genes in Extended Data Fig. 8d. n = 3 per group. p, Hepatic TG content at 12 weeks of HFD. q, Relative mRNA levels of indicated hepatic genes at 4 weeks of HFD. n = 9 (Adipo-Appbp2 KO), n = 10 (Control). r, Serum cholesterol and triglyceride levels in p. a, b, d–r, biologically independent samples. Data are mean ± s.e.m.; two-sided P values by unpaired Student’s t-test (a, b, d–f, h, j, l–r) or two-way repeated-measures ANOVA followed by Fisher’s LSD test (g, i).
