Extended Data Fig. 6: Development of mature CD8+ T cells is largely normal in Mybfl/flCd4Cre mice.
From: MYB orchestrates T cell exhaustion and response to checkpoint inhibition
Adult 8-12 weeks Mybfl/flCd4Cre (Myb-cKO) and littermate Mybfl/fl control (Ctrl) mice were euthanized, and T cell populations were analysed in the thymus, spleen and lymph nodes. Flow cytometry and quantifications showing (a–c) frequencies of thymocyte subsets, (d–f) frequencies and abundance of mature splenic CD8+ T cells, (g) surface expression of CD127 (IL-7R), CCR7 and CD25 (IL-2R) of splenic CD8+ T cells and (h, i) frequencies of mature CD8+ T cells residing in lymph nodes. (h). Dots in graphs represent individual mice; box plots indicate range, interquartile and median. All data are representative of two independent experiments. P values are from two-tailed unpaired t-tests (d, f–h) and Mann–Whitney tests (a, c, e, i); P > 0.05, not significant (n.s.).
