Extended Data Fig. 6: Place field size of Fos-high and Fos-low cells, position decoding and spatial information in activity-matched Fos-high and Fos-low populations, and consistency of Fos induction across stable performance sessions.
From: Fos ensembles encode and shape stable spatial maps in the hippocampus
a) Example place field and description of size measurement. Place field size is quantified as the number of bins above the 99th percentile of the shuffle distribution, multiplied by the width of each bin. b) Scatter of each session’s average place field size for simultaneously recorded Fos-high and Fos-low cells. Fos-high vs. Fos-low: p = 1.1 x 10−9, two-sided paired-sample t-test; n = 27 sessions, 6 mice. c) Activity matching procedure and example session. d) Session-wise scatter and difference histogram of mean integrated ∆F/F per minute in Fos-high and Fos-low populations. After activity-matching, Fos-high vs. Fos Low: p = 0.85, two-sided paired-sample t-test. n = 27 sessions, 6 mice. e) Example activity and decoding performance on held-out test data from activity-matched Fos-high and Fos-low populations. Not all timepoints are necessarily contiguous as train-test split used interleaved trials (Methods). Top and middle: activity of Fos-high and Fos-low cells. Each row is a different cell, and cells are sorted by their preferred position on the track. Bottom: smoothed (gaussian kernel, 0.5 s standard deviation) absolute frame-wise decoding error for Fos-high (red) and Fos-low (blue) decoders. f) Session-wise scatter and difference histogram comparing decoding error. Fos-high vs. Fos-low: p = 5.2 x 10−10, two-sided paired-sample t-test. n = 27 sessions, 6 mice. g) Session-wise scatter and difference histogram comparing fraction of cells with place fields. Fos-high vs. Fos-low: p = 4.9 x 10−11, two-sided paired-sample t-test. n = 27 sessions, 6 mice. h) Session-wise scatter and difference histogram comparing decoding error. Fos-high vs. Fos-low: p = 1.4 x 10−7, two-sided paired-sample t-test. n = 27 sessions, 6 mice. i) Scatter of fold induction values in cells recorded on consecutive sessions. n = 12,645 cells, 18 sessions, 4 mice. j) Similarity in Fos fold induction between session n and session n+1, measured as the Spearman correlation coefficient. Circles indicate the mean for individual sessions, and the vertical line indicates the mean across sessions. Histogram indicates the mean similarity in 1000 permutations of cell identities. p < 0.001. n = 18 sessions from 4 mice. k) Similar to (j), except with similarity in Fos induction measured as Jaccard similarity. p < 0.001.
