Extended Data Fig. 9: Spike-evoked calcium influx and electrophysiological properties of Fos-KO and wildtype neurons.
From: Fos ensembles encode and shape stable spatial maps in the hippocampus
a) Schematic of the experiment. Fosfl/fl;Fosbfl/fl;Junbfl/fl mice were transduced with AAVs to express Cre-GFP in CA1 of the hippocampus (low density). Mice were exposed to an enriched environment for at least two days. b–c) Left, example images and ∆F/F fluorescence traces for Cre+ (Fos-KO) and Cre- (Wt) cells. Red boxes are somatic ROIs used to extract fluorescence traces. Right, example calcium imaging traces. Black ticks mark evoked spikes, numbers indicate number of spikes in a 50 Hz train. d) Average spike-evoked ∆F/F fluorescence for 1, 3, 5, 7, and 9 action potentials. Wt: n = 21 cells, 6 mice. Cre+: n = 24 cells, 6 mice. Mean ± s.e.m. across cells. e) Quantification of peak calcium influx. Wt vs. Cre+: 1 AP p = 0.87; 3 APs p = 0.82; 5 APs p = 0.78; 7 APs p = 0.79; 9 APs p = 0.79; two-sample t-test. Wt: n = 21 cells, 6 mice. Cre+: n = 24 cells, 6 mice. Black squares and error bars denote mean ± s.e.m. f–g) Decay kinetics quantified with double-exponential fit. Wt vs. Cre+: tau slow, p = 0.28; tau fast, p = 0.65; two-sample t-test. Wt: 21 cells, 6 mice. Cre+: 24 cells, 6 mice. Black squares and error bars denote mean ± s.e.m. h–k) Quantification of electrophysiological properties. Wt vs. Cre+: input resistance, p = 0.24; rheobase, p = 0.25; AP amplitude, p = 0.62; AP width, p = 0.10; two-sample t-test: Wt: n = 7 cells, 1 mouse. Cre+: n = 14 cells, 1 mouse. Black squares and error bars denote mean ± s.e.m.
