Extended Data Fig. 1: Muscimol-based inactivation of CA1.
From: Fos ensembles encode and shape stable spatial maps in the hippocampus
a) Schematic of bilateral injections of muscimol or saline into dorsal CA1. For each mouse, sessions alternated between saline and muscimol across days. Adapted, with permission, from Franklin and Paxinos79. b) Performance measured as licking selectivity on alternating muscimol and saline sessions. Each set of connected dots is one mouse. n = 4 mice, 8–10 sessions per mouse. c) Fraction of spatial bins with a lick for saline and muscimol sessions. Each pair of connected dots is a single mouse. p = 0.019, two-sided permutation test, 1000 shuffles. n = 4 mice, 10 sessions per mouse. d) Lick rate for saline and muscimol sessions. Each pair of connected dots is a single mouse. p = 0.17, two-sided permutation test, 1000 shuffles. n = 4 mice, 10 sessions per mouse. e–g) Fraction of trials with a lick, running speed, and fraction of trials with a consumption lick as a function of maze position for saline and muscimol sessions for individual mice. h) Histogram of session average running speed for saline and muscimol sessions. Sessions with mean speeds between 10 and 30 cm/s were chosen for the speed-matched analysis, resulting in 8 saline sessions from 4 mice and 7 muscimol sessions from 2 mice. i) Spatially binned running speed and fraction of trials with a lick for speed-matched saline and muscimol sessions. j) Mean running speed for speed-matched saline and muscimol sessions. Saline vs. muscimol: p = 0.76; two-sample t-test. Error bars, mean ± s.e.m. n = 8 sessions (saline) and 7 sessions (muscimol). k) Mean lick selectivity for speed-matched saline and muscimol sessions. Saline vs. muscimol: p = 3.2 x 10−7; two-sample t-test. Error bars, mean ± s.e.m. n = 8 sessions (saline) and 7 sessions (muscimol).
