Extended Data Fig. 7: PAM-distal positioning and REC lobe conformation in the 10-nt match complex.
From: R-loop formation and conformational activation mechanisms of Cas9
a, Cryo-EM density of the 10-nt match complex overlaid with the structural model. NTS density can be traced along the heteroduplex (white). b, Cartoon representations of the X-ray crystallographic structures of the 10-nt match complex as based on the two complex copies (molecules A and B) in the crystallographic asymmetric unit. The complexes exhibit highly similar conformations (RMSD 0.46 Å). c, Alignment of protein sequences of the REC2 DDD and REC3 RRR helices from REC2-containing Cas9 orthologs.
