Extended Data Fig. 10: NAGK is conserved from prokaryotes to eukaryotes and Drosophila NAGK can operate upstream of NOD2.
From: Phosphorylation of muramyl peptides by NAGK is required for NOD2 activation
a, Cladogram of NAGK proteins across the indicated species in a clockwise fashion (bacteria, archaea, rotifera, nematoda, platyhelminthes, arthropoda, echinodermata, mollusca, porifera, radiata, amphibia, fish, reptilia, aves, and mammalia). Species harbouring NOD2 protein sequences are marked in dark pink. Blue dots represent locations of specific species of interest. b, KBM-7-IL1BmScarlet WT, NAGKKO pool cells or NAGKKO pool cells reconstituted with doxycycline-inducible drosophila NAGK (CG6218) were stimulated as indicated. After 16 h they were analysed by flow cytometry or their supernatant was assessed for IP-10 production. Mean ± s.e.m of n = 4 independent biological samples; two-way ANOVA with Dunnett’s multiple comparisons test. **** p < 0.0001 or as indicated.
