Fig. 2: The three major conformations of class II.i in warm-grown plants.
From: In vivo single-molecule analysis reveals COOLAIR RNA structural diversity
a–c, Representative structural models of warm conformation 1 (a), warm conformation 2 (b) and warm conformation 3 (c) from d. Models were coloured according to the likelihood of single strandedness. The red arrowheads indicate the site corresponding to the FLC TSS. d, Visualization of the in vivo structural conformations of class II.i in warm-grown plants. Structures were directly generated from 3,061 individual mutational profiles. Data were visualized using principal component analysis (PCA). Each dot represents a unique single structure derived from each single-molecule mutational profile. 3WJ, three-way junction; H#, helix number; MWJ, multiway junction; L-turn, left-handed turn motif; PC, principal component; R-turn, right-handed turn motif. Black arrow, COOLAIR exon boundary; red arrowhead, reverse-complementary to the FLC TSS.
