Extended Data Fig. 2: Two classes of NTS neurons respond to gastric distension with different adaptation rates.
a, Heat map depicting the time-resolved responses (color coded based on the percentage increase in ΔF/F of jRGECO1a fluorescence from baseline) of all 81 (1 mouse) responding NTS neurons to ten consecutive gastric distensions (green bars). Each row shows the response of an individual neuron, with rows sorted by response amplitude, white bar: 10 s. b, A rank-ordered plot of peak ΔF/F for all 81 neurons depicted in Fig. 1c, gray circles: peak response amplitudes of individual trials; red circles: means of peak response amplitudes across trials. c, Representative traces depicting normalized ΔF/F over time for individual neurons to repeated stomach stretch (600 μl, distension rate: ~25 μl/s for trials #1 and #3, and ~300 μl/s for trials #2 and #4), scale bar: 10 s. d, A histogram depicting response duration (full width at quarter maximum) of slowly adapting (green) and rapidly adapting (yellow) neurons in Fig. 1c. e, A representative two-photon image of H2B-jRGECO1a fluorescence in the NTS (transverse view) with neurons pseudocolored based on their slow (green) or rapid (yellow) adaptation to stomach stretch, scale bar: 100 μm. f, Positions of neurons that adapt slowly (green, 544 neurons) or rapidly (yellow, 650 neurons) to stomach stretch, with axis origin corresponding to the centroid for slowly adapting neurons, 19 mice, color scale depicts neuron density (see methods), axis length: 200 μm, R: rostral, M: medial. g, Percentage of slow-adapting (green, 289 neurons in 13 mice) and fast-adapting (yellow, 493 neurons in 13 mice) stomach stretch-activated neurons that also responded to duodenum stretch (90, 115, or 140 μl). h, Responses of slow-adapting (green, 316 neurons in 13 mice) and fast-adapting (yellow, 518 neurons in 13 mice) neurons to various magnitudes of gastric distension; maximal ΔF/F normalized to 100% for each neuron, mean ± sem.
