Extended Data Fig. 2: Cryo-EM data processing of PBS data.
From: Structures of a phycobilisome in light-harvesting and photoprotected states
a, Example raw micrograph before and after streptavidin lattice subtraction. b, Fourier transform of a showing Bragg diffraction of streptavidin crystal before subtraction. Tens of thousands of similar micrographs were recorded. c, Processing pipeline of dataset 2, yielding the structures presented in this paper. Blue to red color shadings represent local resolution estimates at FSC = 0.143 ranging from 2 to 6 Å. The three PBS conformations and the structure of the rod are shown at two different thresholds to visualize both overall shape and higher resolution details.
