Extended Data Fig. 1: High-resolution phenotyping of sickness behaviors.
From: Brainstem ADCYAP1+ neurons control multiple aspects of sickness behaviour
a, Schematic of phenotyping cages used for measurement of food intake, water intake, and movement. b, Daily food intake for saline (grey) and LPS doses of 0.1mg/kg (light red), 0.5mg/kg (red), and 2.5mg/kg (dark red). c, P-value statistics from RM two-way ANOVA for each day of food intake. d, 24 h food intake for different hyperphagic models. WT, wildtype; Starved, wildtype animals starved for 24 h; Ob, leptin deficient animals; AgRP, AgRP-cre animals with stereotactic injection of activating DREADDs in the arcuate region stimulated with CNO. Saline injection (grey) and 0.5mg/kg LPS injection (red) is shown for each animal model. LPS inhibited food intake in all four of these animal models. e,f, BAT temperature (TBAT) (e) and tail temperature (Ttail) (f)time course for 48 h after injection of saline (N = 12 and N = 8, grey) or LPS (0.1mg/kg, light red, N = 6 and N = 7; 0.5mg/kg, medium red, N = 9 and N = 7; 2.5mg/kg, dark red, N = 8 and N = 8). g-i, Minimal recorded core (g), BAT (h), and tail temperature (i) for animals from time course experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 and NS, not significant (P > 0.05). All error bars represent s.e.m. The mouse illustration in a was created using BioRender.com.
